This workshop has ended. Access the training materials on Zenodo.

Metabarcoding has revolutionized the study of biodiversity science. By combining DNA taxonomy with high-throughput DNA sequencing, it offers the potential to observe a larger diversity in the taxa within a single sample, rapidly expanding the scope of microbial analysis and generating high-quality biodiversity data. 

This workshop will introduce the topic of metabarcoding and how you can use Qiime2 to analyse 16S data and gain simultaneous identification of all taxa within a sample. Qiime2 is a popular tool used to perform powerful microbiome analysis that can transform your raw data into publication quality visuals and statistics. In this workshop, using example 16S data from the shallow-water marine anemone E. diaphana, you will learn how to use this pipeline to run essential steps in microbial analysis including generating taxonomic assignments and phylogenic trees, and performing both alpha- and beta- diversity analysis. 

Date/time: 12:30 - 5pm AEDT/ 11:30 - 4pm AEST/ 12 - 4:30pm ACDT/ 9:30am - 2pm AWST, Tuesday 22 February 2022

Lead Trainers: Dr Ashley Dungan (School of Bioscience, University of Melbourne) and Dr Gayle Philip (Melbourne Bioinformatics)

Learning outcomes

By the end of this workshop you should be able to:

• Understand data and metadata formats required for Qiime2

• Use Qiime2 to:

  • Create and interpret sequence quality data

  • Generate taxonomy reports/tables and phylogenic trees based on amplicon sequence variants

  • Compare Alpha and Beta data analysis

  • Develop publication quality graphics and statistics using 16S sequencing data

• Assess the results to determine the influence of genotype (an intrinsic factor) and environment (an extrinsic factor) on anemone-associated bacterial communities

​​Who the workshop is for
This workshop is for researchers studying microbiomes as part of their projects.

The workshop will be conducted in a Unix environment so basic command line knowledge (e.g. logging in to a remote machine, navigating the directory structure and copy files between the computers) is a prerequisite

You should be familiar with the concepts of microbial analysis a however no previous experience with Qiime2 is required.

How to apply

This workshop is free but participation is subject to application with selection.

You must be associated with an Australian organisation and provide an appropriate organisational email address for your application to be considered. 

Applications close 8 February 2022. Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application Successful applicants will be provided with a Zoom meeting link closer to the date.

Applications have closed.

This workshop is presented by the Australian BioCommons and Melbourne Bioinformatics with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.

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Cover image: Small microcosm, Michael Schiffer (unsplash)